Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting

SYC Tong; MTG Holden; EK Nickerson; BS Cooper; CU Koser; A Cori; T Jombart; S Cauchemez; C Fraser; V Wuthiekanun; J Thaipadungpanit; M Hongsuwan; NP Day; D Limmathurotsakul; J Parkhill; SJ Peacock

Journal article

Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting

SYC Tong, MTG Holden, EK Nickerson, BS Cooper, CU Koser, A Cori, T Jombart, S Cauchemez, C Fraser, V Wuthiekanun, J Thaipadungpanit, M Hongsuwan, NP Day, D Limmathurotsakul, J Parkhill, SJ Peacock

Genome Research | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | Published : 2015

DOI: 10.1101/gr.174730.114

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infection. Whole-genome sequencing of MRSA has been used to define phylogeny and transmission in well-resourced healthcare settings, yet the greatest burden of nosocomial infection occurs in resource-restricted settings where barriers to transmission are lower. Here, we study the flux and genetic diversity of MRSA on ward and individual patient levels in a hospital where transmission was common. We repeatedly screened all patients on two intensive care units for MRSA carriage over a 3-mo period. All MRSA belonged to multilocus sequence type 239 (ST 239). We defined the population structure and charted the sprea..

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University of Melbourne Researchers

Steven Tong Author

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CLINICAL AND GENOMIC ASPECTS OF STAPHYLOCOCCUS AUREUS AND OTHER INFECTIOUS DISEASES IN NORTHERN AUSTRALIA

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Awarded by Medical Research Council

Funding Acknowledgements

We thank Drs. Pramot Srisamang, Weera Manavanakul, and Manas Kanoksil and the staff at Sappasithiprasong Hospital for their support in the bacterial isolate collection. The authors acknowledge financial support from the UKCRC Translational Infection Research (TIR) Initiative and the Medical Research Council (Grant number G1000803), with contributions to the grant from the Biotechnology and Biological Sciences Research Council, the National Institute for Health Research on behalf of the Department of Health, and the Chief Scientist Office of the Scottish Government Health Directorate (to Professor Peacock); from Wellcome Trust grant number 098051 awarded to the Wellcome Trust Sanger Institute; and the NIHR Cambridge Biomedical Research Centre (to Professor Peacock). S.Y.C.T. is an Australian National Health and Medical Research Council Career Development Fellow (1065736).